Construct: shRNA TRCN0000136014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014631.2-4314s1c1
DNA Barcode:
GCCTTTGGTTTGCGTCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3PXD2A (9644)
Gene Description:
SH3 and PX domains 2A
Transcript:
RefSeq NM_014631.2 (CURRENT)
Match location:
Position 4314 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGGTTTGCGTCTTATT
Hairpin Sequence:
5'-CCGG-GCCTTTGGTTTGCGTCTTATT-CTCGAG-AATAAGACGCAAACCAAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGGTTTGCGTCTTATTCTCGAGAATAAGACGCAAACCAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGGTTTGCGTCTTATTCTCGAGAATAAGACGCAAACCAAAGGC-3'

Other clones with same target sequence:

(none)