Construct: shRNA TRCN0000136017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016495.2-273s1c1
DNA Barcode:
GAAGGTGCTTCTAGGAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D7 (51256)
Gene Description:
TBC1 domain family member 7
Transcript:
RefSeq NM_016495.2 (NON-CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTGCTTCTAGGAATCTT
Hairpin Sequence:
5'-CCGG-GAAGGTGCTTCTAGGAATCTT-CTCGAG-AAGATTCCTAGAAGCACCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTGCTTCTAGGAATCTTCTCGAGAAGATTCCTAGAAGCACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTGCTTCTAGGAATCTTCTCGAGAAGATTCCTAGAAGCACCTTC-3'

Other clones with same target sequence:

(none)