Construct: shRNA TRCN0000136032

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153376.1-1268s1c1
DNA Barcode:
GAGGAACGAAATGAGGAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC96 (257236)
Gene Description:
coiled-coil domain containing 96
Transcript:
RefSeq NM_153376.1 (NON-CURRENT)
Match location:
Position 1268 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136032)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAACGAAATGAGGAACTT
Hairpin Sequence:
5'-CCGG-GAGGAACGAAATGAGGAACTT-CTCGAG-AAGTTCCTCATTTCGTTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAACGAAATGAGGAACTTCTCGAGAAGTTCCTCATTTCGTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAACGAAATGAGGAACTTCTCGAGAAGTTCCTCATTTCGTTCCTC-3'

Other clones with same target sequence:

(none)