Construct: shRNA TRCN0000136054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032269.3-2242s1c1
DNA Barcode:
GAATGAGAAGAGCAAGGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DRC7 (84229)
Gene Description:
dynein regulatory complex subunit 7
Transcript:
RefSeq NM_032269.3 (NON-CURRENT)
Match location:
Position 2242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGAGAAGAGCAAGGAATA
Hairpin Sequence:
5'-CCGG-GAATGAGAAGAGCAAGGAATA-CTCGAG-TATTCCTTGCTCTTCTCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGAGAAGAGCAAGGAATACTCGAGTATTCCTTGCTCTTCTCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGAGAAGAGCAAGGAATACTCGAGTATTCCTTGCTCTTCTCATTC-3'

Other clones with same target sequence:

(none)