Construct: shRNA TRCN0000136161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138423.2-1539s1c1
DNA Barcode:
GATGATGAAGAACGAGAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASC4 (113201)
Gene Description:
cancer susceptibility 4
Transcript:
RefSeq NM_138423.2 (NON-CURRENT)
Match location:
Position 1539 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATGAAGAACGAGAGCTT
Hairpin Sequence:
5'-CCGG-GATGATGAAGAACGAGAGCTT-CTCGAG-AAGCTCTCGTTCTTCATCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATGAAGAACGAGAGCTTCTCGAGAAGCTCTCGTTCTTCATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATGAAGAACGAGAGCTTCTCGAGAAGCTCTCGTTCTTCATCATC-3'

Other clones with same target sequence:

(none)