Construct: shRNA TRCN0000136225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030938.2-1698s1c1
DNA Barcode:
GCTTGTCACAAATGATTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VMP1 (81671)
Gene Description:
vacuole membrane protein 1
Transcript:
RefSeq NM_030938.2 (NON-CURRENT)
Match location:
Position 1698 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTCACAAATGATTGCTT
Hairpin Sequence:
5'-CCGG-GCTTGTCACAAATGATTGCTT-CTCGAG-AAGCAATCATTTGTGACAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTCACAAATGATTGCTTCTCGAGAAGCAATCATTTGTGACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTCACAAATGATTGCTTCTCGAGAAGCAATCATTTGTGACAAGC-3'

Other clones with same target sequence:

(none)