Construct: shRNA TRCN0000136278

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145248.2-287s1c1
DNA Barcode:
GAGAATTATCAAGCTGGTGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPACA7 (122258)
Gene Description:
sperm acrosome associated 7
Transcript:
RefSeq NM_145248.2 (NON-CURRENT)
Match location:
Position 287 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136278)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATTATCAAGCTGGTGGT
Hairpin Sequence:
5'-CCGG-GAGAATTATCAAGCTGGTGGT-CTCGAG-ACCACCAGCTTGATAATTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATTATCAAGCTGGTGGTCTCGAGACCACCAGCTTGATAATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATTATCAAGCTGGTGGTCTCGAGACCACCAGCTTGATAATTCTC-3'

Other clones with same target sequence:

(none)