Construct: shRNA TRCN0000136334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004772.1-1786s1c1
DNA Barcode:
GCCTGAGTTTCTTGTGCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NREP (9315)
Gene Description:
neuronal regeneration related protein
Transcript:
RefSeq NM_004772.1 (NON-CURRENT)
Match location:
Position 1786 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGTTTCTTGTGCATTA
Hairpin Sequence:
5'-CCGG-GCCTGAGTTTCTTGTGCATTA-CTCGAG-TAATGCACAAGAAACTCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGTTTCTTGTGCATTACTCGAGTAATGCACAAGAAACTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGTTTCTTGTGCATTACTCGAGTAATGCACAAGAAACTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000322778