Construct: shRNA TRCN0000136355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201453.1-1186s1c1
DNA Barcode:
GCTACTGTGACAGAAACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBWD3 (445571)
Gene Description:
COBW domain containing 3
Transcript:
RefSeq NM_201453.1 (NON-CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTGTGACAGAAACAGAA
Hairpin Sequence:
5'-CCGG-GCTACTGTGACAGAAACAGAA-CTCGAG-TTCTGTTTCTGTCACAGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTGTGACAGAAACAGAACTCGAGTTCTGTTTCTGTCACAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTGTGACAGAAACAGAACTCGAGTTCTGTTTCTGTCACAGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000130465