Construct: shRNA TRCN0000136391

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018188.2-2067s1c1
DNA Barcode:
CCTGCACATTTAGGATATGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATAD3A (55210)
Gene Description:
ATPase family AAA domain containing 3A
Transcript:
RefSeq NM_018188.2 (NON-CURRENT)
Match location:
Position 2067 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136391)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCACATTTAGGATATGCT
Hairpin Sequence:
5'-CCGG-CCTGCACATTTAGGATATGCT-CTCGAG-AGCATATCCTAAATGTGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCACATTTAGGATATGCTCTCGAGAGCATATCCTAAATGTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCACATTTAGGATATGCTCTCGAGAGCATATCCTAAATGTGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000148730