Construct: shRNA TRCN0000136427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052913.2-4547s1c1
DNA Barcode:
CGTTAGATACTCGGAATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM200A (114801)
Gene Description:
transmembrane protein 200A
Transcript:
RefSeq NM_052913.2 (CURRENT)
Match location:
Position 4547 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTAGATACTCGGAATCAAA
Hairpin Sequence:
5'-CCGG-CGTTAGATACTCGGAATCAAA-CTCGAG-TTTGATTCCGAGTATCTAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTAGATACTCGGAATCAAACTCGAGTTTGATTCCGAGTATCTAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTAGATACTCGGAATCAAACTCGAGTTTGATTCCGAGTATCTAACG-3'

Other clones with same target sequence:

(none)