Construct: shRNA TRCN0000136456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031746.1-472s1c1
DNA Barcode:
CCACGGAAATGAGAGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VSTM4 (196740)
Gene Description:
V-set and transmembrane domain containing 4
Transcript:
RefSeq NM_001031746.1 (NON-CURRENT)
Match location:
Position 472 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGGAAATGAGAGTCATTT
Hairpin Sequence:
5'-CCGG-CCACGGAAATGAGAGTCATTT-CTCGAG-AAATGACTCTCATTTCCGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGGAAATGAGAGTCATTTCTCGAGAAATGACTCTCATTTCCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGGAAATGAGAGTCATTTCTCGAGAAATGACTCTCATTTCCGTGG-3'

Other clones with same target sequence:

(none)