Construct: shRNA TRCN0000136458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012075.1-2384s1c1
DNA Barcode:
CCTGAAGAATGTGCAGAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPRL3 (8131)
Gene Description:
NPR3 like, GATOR1 complex subunit
Transcript:
RefSeq NM_012075.1 (NON-CURRENT)
Match location:
Position 2384 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGAATGTGCAGAAACA
Hairpin Sequence:
5'-CCGG-CCTGAAGAATGTGCAGAAACA-CTCGAG-TGTTTCTGCACATTCTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGAATGTGCAGAAACACTCGAGTGTTTCTGCACATTCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGAATGTGCAGAAACACTCGAGTGTTTCTGCACATTCTTCAGG-3'

Other clones with same target sequence:

(none)