Construct: shRNA TRCN0000136475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182529.1-1281s1c1
DNA Barcode:
CAGAAAGTCTCTAAGCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THAP5 (168451)
Gene Description:
THAP domain containing 5
Transcript:
RefSeq NM_182529.1 (NON-CURRENT)
Match location:
Position 1281 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAAGTCTCTAAGCTACAT
Hairpin Sequence:
5'-CCGG-CAGAAAGTCTCTAAGCTACAT-CTCGAG-ATGTAGCTTAGAGACTTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAAGTCTCTAAGCTACATCTCGAGATGTAGCTTAGAGACTTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAAGTCTCTAAGCTACATCTCGAGATGTAGCTTAGAGACTTTCTG-3'

Other clones with same target sequence:

(none)