Construct: shRNA TRCN0000136495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152864.2-650s1c1
DNA Barcode:
CCTCTCTACCATGTCAATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKAIN4 (128414)
Gene Description:
sodium/potassium transporting ATPase interacting 4
Transcript:
RefSeq NM_152864.2 (NON-CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTACCATGTCAATGAA
Hairpin Sequence:
5'-CCGG-CCTCTCTACCATGTCAATGAA-CTCGAG-TTCATTGACATGGTAGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTACCATGTCAATGAACTCGAGTTCATTGACATGGTAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTACCATGTCAATGAACTCGAGTTCATTGACATGGTAGAGAGG-3'

Other clones with same target sequence:

(none)