Construct: shRNA TRCN0000136514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016284.3-6657s1c1
DNA Barcode:
CCACCTAATTGTATCCAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT1 (23019)
Gene Description:
CCR4-NOT transcription complex subunit 1
Transcript:
RefSeq NM_016284.3 (NON-CURRENT)
Match location:
Position 6657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTAATTGTATCCAGTTA
Hairpin Sequence:
5'-CCGG-CCACCTAATTGTATCCAGTTA-CTCGAG-TAACTGGATACAATTAGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTAATTGTATCCAGTTACTCGAGTAACTGGATACAATTAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTAATTGTATCCAGTTACTCGAGTAACTGGATACAATTAGGTGG-3'

Other clones with same target sequence:

(none)