Construct: shRNA TRCN0000136574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015253.1-1328s1c1
DNA Barcode:
CTGTGCAAGACACTCGTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WSCD1 (23302)
Gene Description:
WSC domain containing 1
Transcript:
RefSeq NM_015253.1 (CURRENT)
Match location:
Position 1328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGCAAGACACTCGTTGTA
Hairpin Sequence:
5'-CCGG-CTGTGCAAGACACTCGTTGTA-CTCGAG-TACAACGAGTGTCTTGCACAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGCAAGACACTCGTTGTACTCGAGTACAACGAGTGTCTTGCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGCAAGACACTCGTTGTACTCGAGTACAACGAGTGTCTTGCACAG-3'

Other clones with same target sequence:

(none)