Construct: shRNA TRCN0000136621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178831.4-1330s1c1
DNA Barcode:
GCAGGATCAATGATGGGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASTOR3 (352954)
Gene Description:
CASTOR family member 3
Transcript:
RefSeq NM_178831.4 (NON-CURRENT)
Match location:
Position 1330 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGATCAATGATGGGATAA
Hairpin Sequence:
5'-CCGG-GCAGGATCAATGATGGGATAA-CTCGAG-TTATCCCATCATTGATCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGATCAATGATGGGATAACTCGAGTTATCCCATCATTGATCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGATCAATGATGGGATAACTCGAGTTATCCCATCATTGATCCTGC-3'

Other clones with same target sequence:

(none)