Construct: shRNA TRCN0000136657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024557.2-594s1c1
DNA Barcode:
GCTACATCAGCTCCGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RIC3 (79608)
Gene Description:
RIC3 acetylcholine receptor chaperone
Transcript:
RefSeq NM_024557.2 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACATCAGCTCCGAGAAAT
Hairpin Sequence:
5'-CCGG-GCTACATCAGCTCCGAGAAAT-CTCGAG-ATTTCTCGGAGCTGATGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACATCAGCTCCGAGAAATCTCGAGATTTCTCGGAGCTGATGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACATCAGCTCCGAGAAATCTCGAGATTTCTCGGAGCTGATGTAGC-3'

Other clones with same target sequence:

(none)