Construct: shRNA TRCN0000136670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017420.1-2853s1c1
DNA Barcode:
GCACCTTACTTGTTCTGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ESCO2 (157570)
Gene Description:
establishment of sister chromatid cohesion N-acetyltransferase 2
Transcript:
RefSeq NM_001017420.1 (NON-CURRENT)
Match location:
Position 2853 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTTACTTGTTCTGAGAT
Hairpin Sequence:
5'-CCGG-GCACCTTACTTGTTCTGAGAT-CTCGAG-ATCTCAGAACAAGTAAGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTTACTTGTTCTGAGATCTCGAGATCTCAGAACAAGTAAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTTACTTGTTCTGAGATCTCGAGATCTCAGAACAAGTAAGGTGC-3'

Other clones with same target sequence:

(none)