Construct: shRNA TRCN0000136683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030806.2-730s1c1
DNA Barcode:
GCAAACATGCACATCTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf21 (81563)
Gene Description:
chromosome 1 open reading frame 21
Transcript:
RefSeq NM_030806.2 (NON-CURRENT)
Match location:
Position 730 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACATGCACATCTCTGAA
Hairpin Sequence:
5'-CCGG-GCAAACATGCACATCTCTGAA-CTCGAG-TTCAGAGATGTGCATGTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACATGCACATCTCTGAACTCGAGTTCAGAGATGTGCATGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACATGCACATCTCTGAACTCGAGTTCAGAGATGTGCATGTTTGC-3'

Other clones with same target sequence:

(none)