Construct: shRNA TRCN0000136717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016930.2-244s1c1
DNA Barcode:
CGCGAAGTGATTTCTCACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STX18 (53407)
Gene Description:
syntaxin 18
Transcript:
RefSeq NM_016930.2 (NON-CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGAAGTGATTTCTCACATT
Hairpin Sequence:
5'-CCGG-CGCGAAGTGATTTCTCACATT-CTCGAG-AATGTGAGAAATCACTTCGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGAAGTGATTTCTCACATTCTCGAGAATGTGAGAAATCACTTCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGAAGTGATTTCTCACATTCTCGAGAATGTGAGAAATCACTTCGCG-3'

Other clones with same target sequence:

  1. TRCN0000319346