Construct: shRNA TRCN0000136735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018462.3-174s1c1
DNA Barcode:
GAGAAATTGACAGCCCTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRK1 (55845)
Gene Description:
BRICK1 subunit of SCAR/WAVE actin nucleating complex
Transcript:
RefSeq NM_018462.3 (NON-CURRENT)
Match location:
Position 174 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAATTGACAGCCCTTGAA
Hairpin Sequence:
5'-CCGG-GAGAAATTGACAGCCCTTGAA-CTCGAG-TTCAAGGGCTGTCAATTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAATTGACAGCCCTTGAACTCGAGTTCAAGGGCTGTCAATTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAATTGACAGCCCTTGAACTCGAGTTCAAGGGCTGTCAATTTCTC-3'

Other clones with same target sequence:

(none)