Construct: shRNA TRCN0000136742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024903.1-395s1c1
DNA Barcode:
GAAAGAGTCCAAGCAGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCA11P (79963)
Gene Description:
ATP binding cassette subfamily A member 11, pseudogene
Transcript:
RefSeq NM_024903.1 (NON-CURRENT)
Match location:
Position 395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAGTCCAAGCAGCAAAT
Hairpin Sequence:
5'-CCGG-GAAAGAGTCCAAGCAGCAAAT-CTCGAG-ATTTGCTGCTTGGACTCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAGTCCAAGCAGCAAATCTCGAGATTTGCTGCTTGGACTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAGTCCAAGCAGCAAATCTCGAGATTTGCTGCTTGGACTCTTTC-3'

Other clones with same target sequence:

(none)