Construct: shRNA TRCN0000136804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173654.1-475s1c1
DNA Barcode:
CCTAATACTCACAGCATTCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EOGT (285203)
Gene Description:
EGF domain specific O-linked N-acetylglucosamine transferase
Transcript:
RefSeq NM_173654.1 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAATACTCACAGCATTCCA
Hairpin Sequence:
5'-CCGG-CCTAATACTCACAGCATTCCA-CTCGAG-TGGAATGCTGTGAGTATTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAATACTCACAGCATTCCACTCGAGTGGAATGCTGTGAGTATTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAATACTCACAGCATTCCACTCGAGTGGAATGCTGTGAGTATTAGG-3'

Other clones with same target sequence:

(none)