Construct: shRNA TRCN0000136822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032352.2-1120s1c1
DNA Barcode:
CCAAATGTAAGTGCCATGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRMS1L (84312)
Gene Description:
BRMS1 like transcriptional repressor
Transcript:
RefSeq NM_032352.2 (NON-CURRENT)
Match location:
Position 1120 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATGTAAGTGCCATGAGA
Hairpin Sequence:
5'-CCGG-CCAAATGTAAGTGCCATGAGA-CTCGAG-TCTCATGGCACTTACATTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATGTAAGTGCCATGAGACTCGAGTCTCATGGCACTTACATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATGTAAGTGCCATGAGACTCGAGTCTCATGGCACTTACATTTGG-3'

Other clones with same target sequence:

(none)