Construct: shRNA TRCN0000136890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_114272.4-213s1c1
DNA Barcode:
CATAGGACAGACTGACAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFT172 (26160)
Gene Description:
intraflagellar transport 172
Transcript:
RefSeq XM_114272.4 (NON-CURRENT)
Match location:
Position 213 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGGACAGACTGACAACAT
Hairpin Sequence:
5'-CCGG-CATAGGACAGACTGACAACAT-CTCGAG-ATGTTGTCAGTCTGTCCTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGGACAGACTGACAACATCTCGAGATGTTGTCAGTCTGTCCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGGACAGACTGACAACATCTCGAGATGTTGTCAGTCTGTCCTATG-3'

Other clones with same target sequence:

(none)