Construct: shRNA TRCN0000136902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152544.1-1055s1c1
DNA Barcode:
CCAGGTGTTCCAAGTTGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT44 (152992)
Gene Description:
tRNA methyltransferase 44 homolog
Transcript:
RefSeq NM_152544.1 (NON-CURRENT)
Match location:
Position 1055 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTGTTCCAAGTTGTGAA
Hairpin Sequence:
5'-CCGG-CCAGGTGTTCCAAGTTGTGAA-CTCGAG-TTCACAACTTGGAACACCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTGTTCCAAGTTGTGAACTCGAGTTCACAACTTGGAACACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTGTTCCAAGTTGTGAACTCGAGTTCACAACTTGGAACACCTGG-3'

Other clones with same target sequence:

(none)