Construct: shRNA TRCN0000136921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014255.3-1041s1c1
DNA Barcode:
CGAACAGATCTTTGTGACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNPY2 (10330)
Gene Description:
canopy FGF signaling regulator 2
Transcript:
RefSeq NM_014255.3 (NON-CURRENT)
Match location:
Position 1041 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAACAGATCTTTGTGACCAT
Hairpin Sequence:
5'-CCGG-CGAACAGATCTTTGTGACCAT-CTCGAG-ATGGTCACAAAGATCTGTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAACAGATCTTTGTGACCATCTCGAGATGGTCACAAAGATCTGTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAACAGATCTTTGTGACCATCTCGAGATGGTCACAAAGATCTGTTCG-3'

Other clones with same target sequence:

(none)