Construct: shRNA TRCN0000136957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024086.2-2785s1c1
DNA Barcode:
CCTGTACTTACCTACTCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
METTL16 (79066)
Gene Description:
methyltransferase like 16
Transcript:
RefSeq NM_024086.2 (NON-CURRENT)
Match location:
Position 2785 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000136957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTACTTACCTACTCACAT
Hairpin Sequence:
5'-CCGG-CCTGTACTTACCTACTCACAT-CTCGAG-ATGTGAGTAGGTAAGTACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTACTTACCTACTCACATCTCGAGATGTGAGTAGGTAAGTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTACTTACCTACTCACATCTCGAGATGTGAGTAGGTAAGTACAGG-3'

Other clones with same target sequence:

(none)