Construct: shRNA TRCN0000137050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006952.3-1256s1c1
DNA Barcode:
CCTTCCAATGCTTCTGTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UPK1B (7348)
Gene Description:
uroplakin 1B
Transcript:
RefSeq NM_006952.3 (NON-CURRENT)
Match location:
Position 1256 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCAATGCTTCTGTTGAT
Hairpin Sequence:
5'-CCGG-CCTTCCAATGCTTCTGTTGAT-CTCGAG-ATCAACAGAAGCATTGGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCAATGCTTCTGTTGATCTCGAGATCAACAGAAGCATTGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCAATGCTTCTGTTGATCTCGAGATCAACAGAAGCATTGGAAGG-3'

Other clones with same target sequence:

(none)