Construct: shRNA TRCN0000137130

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016030.5-2288s1c1
DNA Barcode:
CTTCAACCTGACCACCATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAPPC12 (51112)
Gene Description:
trafficking protein particle complex 12
Transcript:
RefSeq NM_016030.5 (NON-CURRENT)
Match location:
Position 2288 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137130)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAACCTGACCACCATGTA
Hairpin Sequence:
5'-CCGG-CTTCAACCTGACCACCATGTA-CTCGAG-TACATGGTGGTCAGGTTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAACCTGACCACCATGTACTCGAGTACATGGTGGTCAGGTTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAACCTGACCACCATGTACTCGAGTACATGGTGGTCAGGTTGAAG-3'

Other clones with same target sequence:

(none)