Construct: shRNA TRCN0000137144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016148.1-556s1c1
DNA Barcode:
GTTGAAGAAGTTCCTGGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHANK1 (50944)
Gene Description:
SH3 and multiple ankyrin repeat domains 1
Transcript:
RefSeq NM_016148.1 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGAAGAAGTTCCTGGAGTA
Hairpin Sequence:
5'-CCGG-GTTGAAGAAGTTCCTGGAGTA-CTCGAG-TACTCCAGGAACTTCTTCAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGAAGAAGTTCCTGGAGTACTCGAGTACTCCAGGAACTTCTTCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGAAGAAGTTCCTGGAGTACTCGAGTACTCCAGGAACTTCTTCAAC-3'

Other clones with same target sequence:

(none)