Construct: shRNA TRCN0000137162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018072.4-6431s1c1
DNA Barcode:
GCTACCAGAATCCATTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HEATR1 (55127)
Gene Description:
HEAT repeat containing 1
Transcript:
RefSeq NM_018072.4 (NON-CURRENT)
Match location:
Position 6431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACCAGAATCCATTCCTTT
Hairpin Sequence:
5'-CCGG-GCTACCAGAATCCATTCCTTT-CTCGAG-AAAGGAATGGATTCTGGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACCAGAATCCATTCCTTTCTCGAGAAAGGAATGGATTCTGGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACCAGAATCCATTCCTTTCTCGAGAAAGGAATGGATTCTGGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000312492