Construct: shRNA TRCN0000137163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023934.2-394s1c1
DNA Barcode:
GATGGTGCACAGGTTTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FUNDC2 (65991)
Gene Description:
FUN14 domain containing 2
Transcript:
RefSeq NM_023934.2 (NON-CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGTGCACAGGTTTCATAT
Hairpin Sequence:
5'-CCGG-GATGGTGCACAGGTTTCATAT-CTCGAG-ATATGAAACCTGTGCACCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGTGCACAGGTTTCATATCTCGAGATATGAAACCTGTGCACCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGTGCACAGGTTTCATATCTCGAGATATGAAACCTGTGCACCATC-3'

Other clones with same target sequence:

  1. TRCN0000234309