Construct: shRNA TRCN0000137183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203447.1-6642s1c2
DNA Barcode:
GCAAACTTGTAGGAGTACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DOCK8 (81704)
Gene Description:
dedicator of cytokinesis 8
Transcript:
RefSeq NM_203447.1 (NON-CURRENT)
Match location:
Position 6642 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACTTGTAGGAGTACGAA
Hairpin Sequence:
5'-CCGG-GCAAACTTGTAGGAGTACGAA-CTCGAG-TTCGTACTCCTACAAGTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACTTGTAGGAGTACGAACTCGAGTTCGTACTCCTACAAGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACTTGTAGGAGTACGAACTCGAGTTCGTACTCCTACAAGTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000122954