Construct: shRNA TRCN0000137213
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018125.2-2076s1c1
- DNA Barcode:
- GCAGGCTCAGAATAAGGTGTA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ARHGEF10L (55160)
- Gene Description:
- Rho guanine nucleotide exchange factor 10 like
- Transcript:
- RefSeq NM_018125.2 (NON-CURRENT)
- Match location:
- Position 2076 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000137213)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Table Loading...
Sequence Information
- Target Sequence:
- GCAGGCTCAGAATAAGGTGTA
- Hairpin Sequence:
- 5'-CCGG-GCAGGCTCAGAATAAGGTGTA-CTCGAG-TACACCTTATTCTGAGCCTGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGGCTCAGAATAAGGTGTACTCGAGTACACCTTATTCTGAGCCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGGCTCAGAATAAGGTGTACTCGAGTACACCTTATTCTGAGCCTGC-3'