Construct: shRNA TRCN0000137252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198275.1-226s1c1
DNA Barcode:
GCAGCCACACAGTATCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPZL3 (196264)
Gene Description:
myelin protein zero like 3
Transcript:
RefSeq NM_198275.1 (NON-CURRENT)
Match location:
Position 226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCACACAGTATCAATAT
Hairpin Sequence:
5'-CCGG-GCAGCCACACAGTATCAATAT-CTCGAG-ATATTGATACTGTGTGGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCACACAGTATCAATATCTCGAGATATTGATACTGTGTGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCACACAGTATCAATATCTCGAGATATTGATACTGTGTGGCTGC-3'

Other clones with same target sequence:

(none)