Construct: shRNA TRCN0000137312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024657.2-2278s1c1
DNA Barcode:
GAGGCAAATCACATACTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MORC4 (79710)
Gene Description:
MORC family CW-type zinc finger 4
Transcript:
RefSeq NM_024657.2 (NON-CURRENT)
Match location:
Position 2278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCAAATCACATACTGGAT
Hairpin Sequence:
5'-CCGG-GAGGCAAATCACATACTGGAT-CTCGAG-ATCCAGTATGTGATTTGCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCAAATCACATACTGGATCTCGAGATCCAGTATGTGATTTGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCAAATCACATACTGGATCTCGAGATCCAGTATGTGATTTGCCTC-3'

Other clones with same target sequence:

(none)