Construct: shRNA TRCN0000137439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014675.2-3261s1c1
DNA Barcode:
GTCTCTGAAGGAGTCTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CROCC (9696)
Gene Description:
ciliary rootlet coiled-coil, rootletin
Transcript:
RefSeq NM_014675.2 (NON-CURRENT)
Match location:
Position 3261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCTGAAGGAGTCTGAGAA
Hairpin Sequence:
5'-CCGG-GTCTCTGAAGGAGTCTGAGAA-CTCGAG-TTCTCAGACTCCTTCAGAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCTGAAGGAGTCTGAGAACTCGAGTTCTCAGACTCCTTCAGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCTGAAGGAGTCTGAGAACTCGAGTTCTCAGACTCCTTCAGAGAC-3'

Other clones with same target sequence:

(none)