Construct: shRNA TRCN0000137525

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015332.2-2167s1c1
DNA Barcode:
CCTTGGAACTTATGGCCCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDCD3 (23386)
Gene Description:
NudC domain containing 3
Transcript:
RefSeq NM_015332.2 (NON-CURRENT)
Match location:
Position 2167 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137525)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGAACTTATGGCCCAAT
Hairpin Sequence:
5'-CCGG-CCTTGGAACTTATGGCCCAAT-CTCGAG-ATTGGGCCATAAGTTCCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGAACTTATGGCCCAATCTCGAGATTGGGCCATAAGTTCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGAACTTATGGCCCAATCTCGAGATTGGGCCATAAGTTCCAAGG-3'

Other clones with same target sequence:

(none)