Construct: shRNA TRCN0000137601

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032434.2-1108s1c1
DNA Barcode:
CGTTCTGCCAAGATAGCTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF512 (84450)
Gene Description:
zinc finger protein 512
Transcript:
RefSeq NM_032434.2 (NON-CURRENT)
Match location:
Position 1108 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137601)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCTGCCAAGATAGCTGTA
Hairpin Sequence:
5'-CCGG-CGTTCTGCCAAGATAGCTGTA-CTCGAG-TACAGCTATCTTGGCAGAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCTGCCAAGATAGCTGTACTCGAGTACAGCTATCTTGGCAGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCTGCCAAGATAGCTGTACTCGAGTACAGCTATCTTGGCAGAACG-3'

Other clones with same target sequence:

  1. TRCN0000285460