Construct: shRNA TRCN0000137605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207324.1-337s1c1
DNA Barcode:
CAAGCTTGAAGAGTGTGAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPACA6 (147650)
Gene Description:
sperm acrosome associated 6
Transcript:
RefSeq NM_207324.1 (NON-CURRENT)
Match location:
Position 337 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCTTGAAGAGTGTGAGGA
Hairpin Sequence:
5'-CCGG-CAAGCTTGAAGAGTGTGAGGA-CTCGAG-TCCTCACACTCTTCAAGCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCTTGAAGAGTGTGAGGACTCGAGTCCTCACACTCTTCAAGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCTTGAAGAGTGTGAGGACTCGAGTCCTCACACTCTTCAAGCTTG-3'

Other clones with same target sequence:

(none)