Construct: shRNA TRCN0000137725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003663.2-455s1c1
DNA Barcode:
GCTGCATGAAGATGGAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CGGBP1 (8545)
Gene Description:
CGG triplet repeat binding protein 1
Transcript:
RefSeq NM_003663.2 (NON-CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATGAAGATGGAGGAAA
Hairpin Sequence:
5'-CCGG-GCTGCATGAAGATGGAGGAAA-CTCGAG-TTTCCTCCATCTTCATGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATGAAGATGGAGGAAACTCGAGTTTCCTCCATCTTCATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATGAAGATGGAGGAAACTCGAGTTTCCTCCATCTTCATGCAGC-3'

Other clones with same target sequence:

(none)