Construct: shRNA TRCN0000137815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032448.1-2959s1c1
DNA Barcode:
GCTTGCATGAAGAAGGAACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM120B (84498)
Gene Description:
family with sequence similarity 120B
Transcript:
RefSeq NM_032448.1 (NON-CURRENT)
Match location:
Position 2959 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCATGAAGAAGGAACGA
Hairpin Sequence:
5'-CCGG-GCTTGCATGAAGAAGGAACGA-CTCGAG-TCGTTCCTTCTTCATGCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCATGAAGAAGGAACGACTCGAGTCGTTCCTTCTTCATGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCATGAAGAAGGAACGACTCGAGTCGTTCCTTCTTCATGCAAGC-3'

Other clones with same target sequence:

(none)