Construct: shRNA TRCN0000137976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015117.1-224s1c1
DNA Barcode:
GCAAGAAATACTCCCTCGTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3H3 (23144)
Gene Description:
zinc finger CCCH-type containing 3
Transcript:
RefSeq NM_015117.1 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAAATACTCCCTCGTGA
Hairpin Sequence:
5'-CCGG-GCAAGAAATACTCCCTCGTGA-CTCGAG-TCACGAGGGAGTATTTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAAATACTCCCTCGTGACTCGAGTCACGAGGGAGTATTTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAAATACTCCCTCGTGACTCGAGTCACGAGGGAGTATTTCTTGC-3'

Other clones with same target sequence:

(none)