Construct: shRNA TRCN0000137997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014985.1-880s1c1
DNA Barcode:
GCATTGTTATGGGCCTCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CEP152 (22995)
Gene Description:
centrosomal protein 152
Transcript:
RefSeq NM_014985.1 (NON-CURRENT)
Match location:
Position 880 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000137997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGTTATGGGCCTCACAA
Hairpin Sequence:
5'-CCGG-GCATTGTTATGGGCCTCACAA-CTCGAG-TTGTGAGGCCCATAACAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGTTATGGGCCTCACAACTCGAGTTGTGAGGCCCATAACAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGTTATGGGCCTCACAACTCGAGTTGTGAGGCCCATAACAATGC-3'

Other clones with same target sequence:

(none)