Construct: shRNA TRCN0000138100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024554.2-3247s1c1
DNA Barcode:
GCTGTGCCCTTTAATGGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGBD5 (79605)
Gene Description:
piggyBac transposable element derived 5
Transcript:
RefSeq NM_024554.2 (NON-CURRENT)
Match location:
Position 3247 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGCCCTTTAATGGGATT
Hairpin Sequence:
5'-CCGG-GCTGTGCCCTTTAATGGGATT-CTCGAG-AATCCCATTAAAGGGCACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGCCCTTTAATGGGATTCTCGAGAATCCCATTAAAGGGCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGCCCTTTAATGGGATTCTCGAGAATCCCATTAAAGGGCACAGC-3'

Other clones with same target sequence:

(none)