Construct: shRNA TRCN0000138186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018072.4-5477s1c1
DNA Barcode:
CCAGGTGATTCATCTGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HEATR1 (55127)
Gene Description:
HEAT repeat containing 1
Transcript:
RefSeq NM_018072.4 (NON-CURRENT)
Match location:
Position 5477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTGATTCATCTGGAGAA
Hairpin Sequence:
5'-CCGG-CCAGGTGATTCATCTGGAGAA-CTCGAG-TTCTCCAGATGAATCACCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTGATTCATCTGGAGAACTCGAGTTCTCCAGATGAATCACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTGATTCATCTGGAGAACTCGAGTTCTCCAGATGAATCACCTGG-3'

Other clones with same target sequence:

(none)