Construct: shRNA TRCN0000138315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001714.1-1793s1c1
DNA Barcode:
CGGTAGAAACAAGGACCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BICD1 (636)
Gene Description:
BICD cargo adaptor 1
Transcript:
RefSeq NM_001714.1 (NON-CURRENT)
Match location:
Position 1793 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000138315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTAGAAACAAGGACCTCAT
Hairpin Sequence:
5'-CCGG-CGGTAGAAACAAGGACCTCAT-CTCGAG-ATGAGGTCCTTGTTTCTACCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTAGAAACAAGGACCTCATCTCGAGATGAGGTCCTTGTTTCTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTAGAAACAAGGACCTCATCTCGAGATGAGGTCCTTGTTTCTACCG-3'

Other clones with same target sequence:

(none)